Abstract：At present, climate change has brought huge challenges to vegetation and ecosystems. As the Qinghai-Tibet Plateau (QTP) is a sensitive area of global climate change, the dynamic assessment of its ecological vulnerability is very important. In order to better quantify the relative size of the ecological vulnerability of the QTP, this study starts from the background characteristics and dynamic change process of the ecosystem, by fitting the vegetation index net primary productivity (NPP) and the temperature, precipitation and meteorological elements. The coefficients of autocorrelation multiple linear regression are used to construct an ecological vulnerability model from the three dimensions of “exposure-sensitivity-elasticity” to conduct a dynamic assessment of ecological vulnerability. Based on the evaluation results, from 2000 to 2015, the ecologically fragile areas were mainly distributed in the eastern and central areas of the QTP. The ecological fragility of the western region showed obvious discontinuities, with high and low vulnerabilities staggered. The three ecosystems of forest, grassland, and bare land have significant differences in their ecological vulnerability to climate change, showing a clear positive correlation in the three dimensions of exposure, sensitivity, and resilience, but in terms of the contribution rates of the three dimensions. The performance is relatively similar, and the relative relationship between the three dimensions is relatively balanced. The longitude and precipitation of the sample points have a greater impact on ecological vulnerability and its three dimensions, and the impact of precipitation on ecological vulnerability is more significant than that of temperature. This research provides theoretical support for plateau ecological conservation and ecological security under the influence of global climate.